Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ctcf

Cell type

Cell type Class
Blood
Cell type
G1E-ER4+E2
Tissue
Blood
Lineage
cellLine
Description
Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Attributes by original data submitter

Sample

source_name
G1E-ER4+E2 cells
cell type
ES-derived erythroid progenitor cells
chip antibody
CTCF (Millipore 07-729)
average chromatin size as measured between a 100-500 bp window using the agilent bioanalyzer 7500 chip
178 bp

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were prepared from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's TruSeq ChIP-seq Sample Preparation Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
31145724
Reads aligned (%)
96.2
Duplicates removed (%)
28.5
Number of peaks
1344 (qval < 1E-05)

mm9

Number of total reads
31145724
Reads aligned (%)
96.0
Duplicates removed (%)
28.5
Number of peaks
796 (qval < 1E-05)

Base call quality data from DBCLS SRA