Antigen

Antigen Class

TFs and others

Antigen

Ctcf

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4+E2 cells

cell type

ES-derived erythroid progenitor cells

chip antibody

CTCF (Millipore 07-729)

average chromatin size as measured between a 100-500 bp window using the agilent bioanalyzer 7500 chip

178 bp

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's TruSeq ChIP-seq Sample Preparation Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

31145724

Reads aligned (%)

96.2

Duplicates removed (%)

28.5

Number of peaks

1344 (qval < 1E-05)

mm9

Number of total reads

31145724

Reads aligned (%)

96.0

Duplicates removed (%)

28.5

Number of peaks

796 (qval < 1E-05)